I-FMOC-Arg(Pbf)-OH (CAS# 154445-77-9)
Ubungozi Nokuphepha
| Izimpawu Eziyingozi | Xi - Irritant |
| Amakhodi Engozi | 36/37/38 - Ukucasula amehlo, uhlelo lokuphefumula kanye nesikhumba. |
| Incazelo Yokuphepha | S26 – Uma uthintana namehlo, geza ngokushesha ngamanzi amaningi futhi ufune iseluleko sezokwelapha. S36 – Gqoka izingubo ezifanele zokuzivikela. |
| I-WGK Germany | 1 |
| Ikhodi ye-HS | 2935 90 90 |
| I-Hazard Class | I-IRRITANT |
Isingeniso
Iqembu le-FOC-protecting liyiqembu elivame ukusetshenziswa elivikela ama-amino acid elivikela iqembu elisebenzayo le-amino le-arginine. Okulandelayo yisingeniso kwezinye zezakhiwo, ukusetshenziswa, izindlela zokulungiselela, kanye nolwazi lokuphepha lwe-Fmoc-Protective Radical:
Ikhwalithi:
Iqembu le-FOC-protecting liyiqembu elivikelayo elikhiphekayo elivikela amaqembu e-amino amino. Ingasabela neqembu lama-amino ku-arginine ngokusabela kwe-esterification ukuze yakhe i-Fmoc-arginine ester, ukuze kufezwe injongo yokuvikela iqembu lama-amino. Kunamaqembu anephunga elimnandi kumolekyuli yeqembu elivikela i-Fmoc elimunca kakhulu ukukhanya kwe-UV, okuvumela ukususwa kweqembu elivikelayo le-Fmoc ukuthi kwenziwe ngemisebe ye-UV noma izindlela zamakhemikhali.
Sebenzisa:
Amaqembu avikela i-FMOC asetshenziswa kakhulu ekuhlanganiseni kwe-peptide kanye nokuhlanganiswa kwesigaba esiqinile. Ingakwazi ukuvikela ngempumelelo iqembu le-arginine amino ukuvimbela ukusabela kwayo ohlangothini ngesikhathi sokuhlanganiswa. Ekuhlanganiseni kwe-peptide, iqembu lokuvikela i-Fmoc lingasuswa yizimo ze-alkaline, okuvumela ukuhlanganiswa kwama-polypeptides ukuthi kuqhubeke.
Indlela:
Iqembu lokuvikela i-Fmoc lingalungiswa ngokusabela kwe-Fmoc-Cl ne-arginine. I-Fmoc-Cl iyi-reagent ene-asidi eqinile ehlangana neqembu le-amino ku-arginine yakhe i-Fmoc-arginine ester. Ukusabela kuvame ukwenziwa nge-ethanol ekamelweni lokushisa kuya ekushiseni kweqhwa lokugeza.
Ulwazi Lokuphepha:
I-FMOC-Protective Radicals iphephile ukusetshenziswa ngaphansi kwezimo ezijwayelekile zaselabhorethri, kodwa okulandelayo kufanele kuqashelwe:
- I-FMOC-CL iyi-ejenti ecasulayo futhi enobuthi, kufanele kuqashelwe ukugwema ukuthintana nesikhumba, ukuhogela noma ukungenwa. Sebenza endaweni engenawo umoya futhi ugqoke izinto zokuzivikela ezifanele.
- I-FMOC-Protective Base inendawo yokumuncwa okuqinile kwemisebe ye-ultraviolet, ngakho-ke kufanele kuthathwe ukunakekelwa ukuze kuvinjelwe ukuthintana okuqondile nesikhumba namehlo ngesikhathi sokusetshenziswa, futhi kude nemithombo yokukhanya enamandla.
- Ukuvikelwa okunamandla kwe-acid hydrolysis njenge-pentafluorophenylcarboxylic acid (TFA) ivame ukusetshenziswa ngesikhathi sokususwa kwamaqembu e-Fmoc-protective, futhi kuyadingeka ukuqaphela ukuthi umhwamuko we-TFA ungabangela umonakalo, ngakho-ke kuyadingeka ukuthi usebenze emthonjeni- indawo engenisa umoya.
