I-fmoc-O-tert-butyl-D-tyrosine (CAS# 118488-18-9)
I-Fmoc-O-tert-butyl-D-tyrosine iyi-amino acid evikelwe evame ukusetshenziswa. Okulandelayo yincazelo yemvelo yayo, ukusetshenziswa, ukwakhiwa kanye nolwazi lokuphepha:
Imvelo:
I-Fmoc-O-tert-butyl-D-tyrosine iyikristale eqinile emhlophe. Kuyinhlanganisela ephilayo enefomula yamakhemikhali ethi C30H31NO7 kanye nesisindo samangqamuzana esingu-521.57g/mol. I-compound iphuma ku-tyrosine lapho iqembu le-amino liphethe iqembu elivikelayo le-Fmoc (9-fluorofluorenylformyl) futhi iqembu le-carboxylic acid lifakwa esterified nge-O-tert-butyl.
Sebenzisa:
I-Fmoc-O-tert-butyl-D-tyrosine ivamise ukusetshenziswa njenge-amino acid evikelwe ekuhlanganiseni kwe-peptide. Ngokunamathisela iqembu elivikelayo le-Fmoc eqenjini le-amino, ukusabela okungafuneki ohlangothini kungavinjelwa ngesikhathi sokuhlanganiswa. Isetshenziswa kabanzi ekuhlanganiseni kwesigaba esiqinile futhi ingasetshenziswa ukwenza ama-polypeptides namaprotheni.
Indlela Yokulungiselela:
Ukulungiswa kwe-Fmoc-O-tert-butyl-D-tyrosine ngokuvamile kwenziwa ngokuhlangana kwamakhemikhali. Okokuqala, i-tyrosine isabela nge-Fmoc-Cl (9-fluorofluorenylcarbonyl chloride) ukukhiqiza i-Fmoc-O-tyrosine. I-cesium tert-butyl bromide ibe isifakwa ekuphenduleni ukuze i-esterify iqembu le-carboxylic acid ukuze lenze i-Fmoc-O-tert-butyl-D-tyrosine. Ekugcineni, umkhiqizo ohlanzekile utholakala ngezinyathelo ze-crystallization, ukugeza nokomisa.
Ulwazi Lokuphepha:
I-Fmoc-O-tert-butyl-D-tyrosine iyinhlanganisela ezinzile ngaphansi kwezimo ezivamile futhi ayinakho ukuguquguquka okusobala ekamelweni lokushisa. Ngesikhathi sokusetshenziswa, kuyadingeka ukuthi uhambisane nezinqubo zokuphepha zaselabhorethri, ugqoke izinto zokuzivikela ezifanele, futhi ugweme ukuthintana nesikhumba namehlo. Lapho uphathwa noma ugcinwa, kufanele ugcinwe endaweni eyomile, epholile futhi kude nomlilo kanye nama-oxidizing agents. Ngesikhathi esifanayo, ukuthintana nama-oxidants aqinile nama-acids aqinile kufanele kugwenywe ukuvimbela ukusabela okuyingozi. Funa usizo lwezokwelapha olusheshayo uma ukumunca noma ukuchayeka ngephutha kwenhlanganisela.
