fmoc-L-4-hydroxyproline (CAS# 88050-17-3)

Isingeniso

I-Fmoc-L-hydroxyproline (Fmoc-Hyp-OH) iphuma ku-amino acid enezici ezilandelayo kanye nokusetshenziswa:

Ikhwalithi:

- Ukubukeka: I-crystalline powder emhlophe noma emhlophe

- I-Solubility: Incibilika kuzincibilikisi eziphilayo njenge-DMF, i-DMSO ne-methanol

Inani le-pKa: 2.76

Sebenzisa:

- I-Fmoc-Hyp-OH isetshenziselwa ikakhulukazi ukuhlanganiswa kwe-peptide kanye nokuhlanganiswa kwe-peptide ekuhlanganiseni kwesigaba esiqinile

- Isebenza njengengxenye yeqembu elivikelayo ukuvikela amaqembu asebenzayo eketango lama-amino acid ngesikhathi sokuhlanganiswa kwesigaba esiqinile ukugwema ukusabela okungalindelekile nokugcina ukukhetha.

Indlela:

I-Fmoc-Hyp-OH ingalungiselelwa ngokuphendula ama-Fmoc-amino acids nge-L-hydroxyproline ku-solvent efanele. Izimo zokusabela ngokuvamile zihlanganisa izinga lokushisa elifanele lokusabela kanye ne-base catalyst efanelekile, njenge-N,N-dimethylpyrrolidone (DMAP). Umkhiqizo owumphumela uhlanzwa ngezinyathelo ezifana nemvula, ukuwasha, nokomisa.

Ulwazi Lokuphepha:

- I-FMOC-HYP-OH iyinhlanganisela yezinto eziphilayo futhi kufanele isingathwe ngokuhambisana nezimiso zokuphepha zaselabhorethri.

- Uthuli lungahogela bese luhlangana nesikhumba, ngakho-ke kufanele kuqashelwe ukugwema ukuhogela ngokuqondile noma ukuthintana.

- Ngesikhathi senqubo, izinto zokuzivikela ezifanele ezifana namagilavu ​​aselabhorethri, ukuvikela amehlo, izingubo zokuzivikela, njll. kufanele kugqokwe.

- Kufanele igcinwe ivalwe ngokuqinile endaweni eyomile, epholile, kude nomlilo nezinto ezivuthayo.