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I-Isopropyl-beta-D-thiogalactopyranoside (CAS#367-93-1) Isithombe Esifakiwe
  • I-Isopropyl-beta-D-thiogalactopyranoside (CAS#367-93-1) 1

Isopropyl-beta-D-thiogalactopyranoside (CAS#367-93-1)

Isakhiwo Samakhemikhali:

I-Molecular Formula I-C9H18O5S
I-Molar Mass 238.3
Ukuminyana 1.3329 (isilinganiso esinzima)
I-Melting Point 105 °C
I-Boling Point 350.9°C (isilinganiso esinzima)
Ukuzungezisa Okuthile(α) -31 º (c=1, amanzi)
I-Flash Point 219°C
Ukuncibilika kwamanzi encibilikayo
Ukuncibilika Incibilika emanzini, ne-methanol
I-Vapor Presure 1.58E-09mmHg ku-25°C
Ukubukeka Impushana emhlophe
Umbala Emhlophe
I-Merck 14,5082
I-BRN 4631
pKa 13.00±0.70(Okubikezelwe)
Isimo Sesitoreji 2-8°C
Ukuzinza Izinzile. Akuhambisani nama-oxidizing agents aqinile.
Kuyazwela `Kuzwela` kumswakama nokushisa
I-Refractive Index 1.5060 (isilinganiso)
I-MDL I-MFCD00063273

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Ubungozi Nokuphepha

Amakhodi Engozi I-R19 – Ingase yenze ama-peroxides aqhumayo
R40 – Ubufakazi obuncane bomphumela we-carcinogenic
R66 – Ukuchayeka kaningi kungabangela ukomisa kwesikhumba noma ukuqhekeka
R36/37/38 – Ukucasula amehlo, uhlelo lokuphefumula kanye nesikhumba.
Incazelo Yokuphepha S23 – Ungawuphefumuli umhwamuko.
I-S24/25 - Gwema ukuthintana nesikhumba namehlo.
I-S36/37 – Gqoka izingubo zokuzivikela ezifanele namagilavu.
S22 - Ungaphefumuli uthuli.
S36/37/39 – Gqoka izingubo zokuzivikela ezifanele, amagilavu ​​kanye nokuvikela iso/ubuso.
S27 – Khumula ngokushesha zonke izingubo ezingcolile.
S26 – Uma uthintana namehlo, geza ngokushesha ngamanzi amaningi futhi ufune iseluleko sezokwelapha.
I-WGK Germany 3
AMAKHODI E-FLUKA BRAND F 10
I-TSCA Yebo
Ikhodi ye-HS 29389090

 

 

Isingeniso

I-IPTG iyinto eyenza umsebenzi we-β-galactosidase. Ngokusekelwe kulesi sici, lapho i-DNA yevekhtha yochungechunge lwe-pUC (noma enye i-DNA yevekhtha enofuzo lwe-lacZ) iguqulwa ngamaseli okususa i-lacZ njengomsingathi, noma lapho i-DNA yevekhtha ye-M13 phage idluliselwa, uma i-X-gal ne-IPTG yengezwa. endaweni yepuleti, ngenxa ye-α-complementarity ye-β-galactosidase, i-recombinant yofuzo ingakhethwa kalula ngokuya ngokuthi amakoloni amhlophe (noma ama-plaque) avela. Ngaphezu kwalokho, ingasetshenziswa futhi njenge-inducer yenkulumo yamavekhtha enkulumo enabathuthukisi abafana ne-lac noma i-tac. Incibilika emanzini, i-methanol, i-ethanol, i-acetone, i-chloroform, ayincibiliki ku-ether. I-inducer ye-β-galactosidase ne-β-galactosidase. Ayina-hydrolyzed nge-β-galactoside. Isixazululo se-substrate se-thiogalactosyltransferase. Kwakhiwe: I-IPTG ichithwa emanzini, bese ifakwa inzalo ukuze kulungiswe isixazululo sokugcina (0 · 1M). Ukugxiliswa kwe-IPTG yokugcina epuleti lesikhombisi kufanele kube ngu-0 · 2mM.
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