I-N-Boc-N'-tosyl-D-histidine (CAS# 69541-68-0)

Isingeniso

I-Boc-D-His(Tos)-OH(Boc-D-His(Tos)-OH) isakhi samakhemikhali esinezici ezilandelayo:

Imvelo:

1. Ukubukeka: okuqinile okumhlophe

2. ifomula yamangqamuzana: C21H25N3O6S

3. isisindo samangqamuzana: 443.51

4. Iphuzu lokuncibilika: cishe ku-110-115°C

Sebenzisa:

I-Boc-D-His(Tos)-OH ivame ukusetshenziswa ku-organic synthesis njengeqembu elivikelayo le-D-histidine (D-His). Ukusetshenziswa kwayo okuyinhloko kuhlanganisa:

1. Ekuhlanganisweni kwe-polypeptide, isetshenziselwa ukuhlanganiswa kwe-polypeptide equkethe iqembu elivikelayo le-D-histidine.

2. Isetshenziswa njengendawo emaphakathi emkhakheni wekhemikhali yezokwelapha ekuhlanganisweni kwezinhlanganisela ezihlosiwe zebhayoloji.

3. Ukuklama kanye nokuhlanganiswa kwezinhlanganisela zekhandidethi yezidakamizwa ocwaningweni.

Indlela Yokulungiselela:

Ukulungiswa kwe-Boc-D-His(Tos)-OH kuvame ukwenziwa ngalezi zinyathelo ezilandelayo:

1. I-D-histidine (D-His) yaphendulwa nge-p-toluenesulfonyl chloride (p-toluenesulfonyl chloride) ukunikeza i-D-histidine p-toluenesulfonyl (D-His-Tos).

2. Ngaphansi kwezimo ze-alkaline, i-D-His-Tos isabela nge-n-butoxycarbonylhydrazine (tert-butyloxycarbonylhydrazide) ukuze kukhiqizwe i-Boc-D-His(Tos)-OH.

Ulwazi Lokuphepha:

Ukuhlolwa kokuphepha kwe-Boc-D-His(Tos)-OH kukhawulelwe, kodwa njengekhemikhali, ukucatshangelwa kokuphepha okulandelayo kufanele kuqashelwe:

1. Gwema ukuhogela, ukuthintana nesikhumba namehlo. Gqoka izinto zokuzivikela ezifanele, njengamagilavu ​​nezibuko.

2. Imikhuba emihle yaselabhorethri kufanele ilandelwe ngesikhathi sokusetshenziswa kanye nezimo ezinhle zokungenisa umoya kufanele kuqinisekiswe.

3. Gcina esitsheni esivaliwe, kude nomlilo kanye ne-oxidant.

4. Landela imithethonqubo kanye neziqondiso ezifanele ngesikhathi sokucubungula.

Uma usebenzisa i-Boc-D-His(Tos)-OH, sicela uqiniseke ukuthi ulandela imithetho efanele yokuphepha yaselabhorethri nemiyalelo yokusebenza, futhi uwagcine futhi uwaphathe kahle amakhemikhali. Uma kunesidingo, kunconywa ukusebenza ngaphansi kokuqondisa kochwepheshe.