I-NN-Bis 9-fluorenylmethyloxycarbonyl-L-histidine CAS 98929-98-7

Incazelo Yokuphepha	S22 - Ungaphefumuli uthuli. I-S24/25 - Gwema ukuthintana nesikhumba namehlo.
Ikhodi ye-HS	29242990

I-N(alpha),N(im)-di-fmoc-L-histidine(N(alpha),N(im)-di-fmoc-L-histidine) iyinhlanganisela etholwe ukusabela kwamakhemikhali kwe-9-fluorenecinol kanye ne-L. - histidine. Ifomula yayo yamakhemikhali yi-C42H38N4O8 futhi isisindo sayo esihlobene ne-molecular mass 722.78g/mol.N(alpha),N(im)-di-fmoc-L-histidine iyinhlanganisela evikela ama-amino acid futhi isetshenziswa kakhulu ekuhlanganiseni kwe-organic kanye nokusabela kwamakhemikhali e-biochemical. . Ngokuyinhloko isetshenziselwa ukulawula ukwakheka nokusebenza kwamaprotheni, kanye nokwandisa nokuthuthukisa uhla lokusetshenziswa kwamaketanga e-polypeptide. Le nhlanganisela isetshenziswa kakhulu kumasu okuhlanganiswa kwesigaba esiqinile sokuguqula i-N-terminus nezinsalela ze-histidine ze-peptide nama-protein molecule.

Indlela yokulungiselela i-N(alpha),N(im)-di-fmoc-L-histidine ivamise ukubandakanya izinyathelo ezintathu. Okokuqala, i-ethylene glycol dimethyl ether ne-diazotoluene zasabela ngaphansi kwe-catalysis ye-cuprous chloride ukuze kuhlanganiswe i-9-fluorenmethanol. Bese, i-9-fluorenecinol ne-L-histidine zisabela ngaphansi kwezimo ezine-acidic ukuze kutholwe i-N(alpha),N(im)-di-fmoc-L-histidine. Ekugcineni, umkhiqizo ohlanzekile utholakala ngezinyathelo ze-crystallization kanye nokuhlanza.

Mayelana nolwazi lokuphepha, i-N(alpha),N(im)-Ayikho imibiko eminingi yocwaningo efanele mayelana nokuphepha okuqondile kwe-di-fmoc-L-histidine, ngakho ukuqapha kuyadingeka. Lapho kusetshenziswa elabhorethri, kufanele kuthathwe izinyathelo ezifanele zokuphepha, okuhlanganisa ukugqoka izinto zokuzivikela ezifanele, njengamagilavu ​​aselabhorethri kanye nezibuko, nokugwema ukuthinta isikhumba namehlo. Ngesikhathi esifanayo, kufanele igcinwe esitsheni esomile, esingena umoya futhi esivaliwe, kude nomlilo nezinto ezivuthayo. Ukuze uthole ulwazi oluningiliziwe lokuphepha, kunconywa ukuthi uthintane nezincwadi ezifanele noma uxhumane nochwepheshe.